Brand: Green Spring
Min. Order: 1
Nitrofuran ELISA Test Kit
diagnostic kits
food safety
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of AOZ (3-anmino-2-oxazolidinone) in the meat, chicken, fish, shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The AOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with AOZ concentration in the sample. This value is compared to the standard curve and the content of the corresponding AOZ is subsequently obtained.

2. Technical specifications
Sensitivity : 0.05 ppb
Detection limit:
Tissue and honey 0.1 ppb
Shrimp,fish(some interference) 0.2 ppb
Recovery rate:
Shrimp and fish 90±10%
Honey ,chicken meat /liver 75±15%
Cross-reaction rate:
AOZ 100%
AHD <0.1%
AMOZ <0.1%
SEM <0.1%

1）3. Components
1. Micro-well strips: 12 strips with 8 removable wells each
2. 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb
3. Enzyme conjugate (12 mL) red cap
4. Antibody working solution (7 mL) blue cap
5. Substrate A solution (7 mL) white cap
6. Substrate B solution (7 mL) black cap
7. Stop solution (7 mL) yellow cap
8. 20× concentrated washing buffer (40 mL) white cap
9. 2× concentrated redissolving solution (50 mL) transparent cap
10. 2-Nitrobenzaldehyde(C7H5NO3)( 15.1 mg) white cap