Brand: Green Spring
Min. Order: 1
Ampicillin ELISA Test Kit
diagnostic kits
Ampicillin
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Ampicillin in the sample. The antigens conjugated Ampicillin is pre-coated on the micro-well stripes, Ampicillin in the sample and the conjugated antigens pre-coated on the micro-well stripes compete for the anti-Ampicillin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Ampicillin concentration in the sample. This value is compared to the standard curve and concentration of Ampicillin in the sample is subsequently obtained.

2. Technical specifications
Sensitivity : 0.1 ppb
Detection limit:
Chicken, duck, pork,liver, fish, shrimp 2 ppb
Honey, milk 2 ppb
Recovery rate:
Chicken, duck, pork,liver, fish, shrimp 75%±10%
Honey, milk 70%±10%
Cross-reaction rate:
Ampenicillin 100%
Benzyl penicillin 0.8%
Cloxacillin 0.2%
Dicloxacillin 0.1%
Amoxicillin 0.1%
Ceftiofu 0.1%

3. Components
1） Micro-well strips: 12 strips with 8 removable wells each
2） 1× standard solution (5 mL) 8.1 ppb white cap
3） Enzyme conjugate (12 mL) red cap
4） Antibody working solution (7 mL) blue cap
5） Substrate A solution (7 mL) white cap
6） Substrate B solution (7 mL) black cap
7） Stop solution (7 mL) yellow cap
8） 20× concentrated washing buffer (40 mL) white cap
9） 20× concentrated redissolving solution (50 mL) transparent cap