Brand: Green spring
Model: 96wells
Min. Order: 1set
Melamine ELISA Test Kit
Melamine Test Kit
Melamine elisa kit
Principle

The MelamineELISA Test Kit is a competitive enzyme-labeled
immunoassay. Melamine is extracted from a sample by blending or
shaking with extraction solution. The Melamine HRP conjugate, sample
extract and calibrators are pipetted into the test wells coated with
Melamine antibody to initiate the reaction. During the 30 minute
incubation period, Melamine from the sample and Melamine HRP
conjugate compete for binding to Melamine antibody. Following this
30 minute incubation, the contents of the well are removed and the
wells are washed to remove any unbound Melamine, and Melamine HRP
conjugate. After wash with laboratory grade water, a clear
substrate is then added to the wells and any bound enzyme conjugate
causes the conversion to a blue color. Following a 30 minute
incubation, the reaction is stopped and the amount of color in each
well is read. The color of the unknown samples is compared to the
color of the calibrators and the Melamine concentration of the
samples is derived.
Specifications

Plate containing 12 test strips of 8 wells each vacuum-packed in
aluminized pouch with indicating dessicant.
4 vials each containing 2 mL of Melamine calibrators corresponding
to 0, 20, 100 and 500 µg/L (ppb) of Melamine.
1 vial containing 7 mL Melamine HRP Enzyme Conjugate.
1 vial containing 14 mL of Substrate.
1 vial containing 14 mL of Stop Solution. (Caution! 1N HCl. Handle
with care.)